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Citations Publications citing this paper. Simultaneous capture and sequential detection of two malarial biomarkers on magnetic microparticles Christine F. Brooks , Charles R Mace. Application of magnetite nanoparticles for the development of highly sensitive immunochromatographic test systems for mycotoxin detection Alina V. Petrakova , Alexandr E. References Publications referenced by this paper.

Lateral Flow Immunoassays - Cytodiagnostics

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Issue 10, Previous Article Next Article. From the journal: Analyst. You have access to this article. Please wait while we load your content Similar to the results of human serum samples, the optical intensities were also correlated with the concentration of each synovial fluid sample, and the optical intensities of our sLFIA device were higher than those by the conventional LFA by 1. Recoveries were calculated by the comparison between different CRP levels measured by Hitachi LST and the intensity obtained from spiked CRP assay in human serum and synovial fluid samples.

In the case of low concentration samples, CRP was undetected thus the recovery was not analyzed. The results indicate that the increased matrix effect and viscosity can extend the duration of the flow time, which can increase the binding time of CRP and AuNP-anti-CRP, thus enhance the optical intensity. Our sLFIA device once again proved to be satisfactory for detection of these kinds of samples. The enhanced-sensitivity of the sLFIA device could potentially be used to quickly diagnose or reassure an individual about their infection status, and the simple design could be widely used as a clinical pre-screening kit.

Not only does the stacking pad extend the interaction time but also increases the interaction probability between the target analyte and AuNP-labeled antibodies, thus enhancing the test performance. In addition to serum and synovial fluid samples, the stacking pad can be widely applied to other types of sample matrices, such as urine, and sweat, which feature lower viscosity than synovial fluid. Additionally, various signal enhancement strategies, such as adding silver enhancement to the AuNPs or using fluorescent nanomaterials as the signal labels of detection antibodies, can be further adopted to enhance the detection limit of the platform 49 , We used ultrapure water The sample pad glass fiber membrane SB08 , absorbent pad SC2 , adhesive backing card, and stacking pads composed of polyester SP1 0.

Shanghai, China. AuNPs were synthesized using the Turkevich method To remove any excess antibodies, 0.

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  • Upon removal of the supernatant, the final conjugates were reconstituted in 0. To prepare the conjugate pads for the Protein A and CRP assay, the conjugates were first diluted to 0. Compared with the traditional LFIA strip, which is composed of the sample pad, the conjugate pad, the nitrocellulose membrane-based test pad, and the absorbent pad Fig. A capture antibody, which recognizes and captures Protein A or CRP on the T-line was dispensed and dried on the test pad.

    The different stacking pads were also characterized with SEM to determine the structure and composition of the materials. Peeling, R. Rapid tests for sexually transmitted infections STIs : the way forward. Wu, J. Biomedical and clinical applications of immunoassays and immunosensors for tumor markers. Trends Anal. Baryeh, K. Development of quantitative immunochromatographic assay for rapid and sensitive detection of carbohydrate antigen CA in human plasma. Kong, M. Lateral flow assay-based bacterial detection using engineered cell wall binding domains of a phage endolysin.

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    Kulabhusan, P. Field-usable lateral flow immunoassay for the rapid detection of white spot syndrome virus WSSV. PloS One 12 , e Seidel, C. Development of a nucleic acid lateral flow immunoassay NALFIA for reliable, simple and rapid detection of the methicillin resistance genes mecA and mecC. Guo, Y. Gold immunochromatographic assay for simultaneous detection of carbofuran and triazophos in water samples. Posthuma-Trumpie, G. Lateral flow immuno assay: its strengths, weaknesses, opportunities and threats. A literature survey. Silver and gold enhancement methods for lateral flow immunoassays.

    Talanta , — Anfossi, L. Increased sensitivity of lateral flow immunoassay for ochratoxin A through silver enhancement. Tsai, T. Antibacterial cellulose paper made with silver-coated gold nanoparticles. Parolo, C. Enhanced lateral flow immunoassay using gold nanoparticles loaded with enzymes.

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    Choi, D. A dual gold nanoparticle conjugate-based lateral flow assay LFA method for the analysis of troponin I. Zhu, M. Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes. Taranova, N. Bifunctional gold nanoparticles as an agglomeration-enhancing tool for highly sensitive lateral flow tests: a case study with procalcitonin.

    Acta , — Tang, R. Improved sensitivity of lateral flow assay using paper-based sample concentration technique.

    Supplementary files

    Fu, E. Enhanced sensitivity of lateral flow tests using a two-dimensional paper network format. Lutz, B. Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper.

    Lateral Flow Webinar: A Guide to Lateral Flow Immunoassay Development

    Lab Chip 11 , — Chemical signal amplification in two-dimensional paper networks. Actuator B-Chem , — Han, K. Three-dimensional paper-based slip device for one-step point-of-care testing. Liu, H. Three-dimensional paper microfluidic devices assembled using the principles of origami.

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    Lateral Flow Immunoassay

    Oh, Y. Vertical flow immunoassay VFA biosensor for a rapid one-step immunoassay. Lab Chip 13 , — Chrambach, A. Polyacrylamide gel electrophoresis. Science , — Shi, Q. One-dimensional polyacrylamide gel electrophoresis. Gel electrophoresis of proteins: A practical approach, 3rd ed. Oxford University Press, Oxford, 1—52 Blackshear, P.

    Systems for polyacrylamide gel electrophoresis.